今日发现的几个小工具，实际使用效果展示
1. ChatPDF网站
网址是：https://www.chatpdf.com/
用户自己上传PDF，上传成功后即可开始问它问题。这里以我问的几个问题进行演示：
问：show me how to get DEGs and immune-related model
答：To get DEGs (differentially expressed genes), you can perform a differential gene expression analysis using tools such as DESeq2, edgeR, or limma. These tools will allow you to compare gene expression levels between different groups (e.g., COVID-19 cases vs. controls) and identify genes that are significantly upregulated or downregulated in one group compared to the other. Once you have identified DEGs, you can use them to build an immune-related model. This can be done using various machine learning algorithms, such as random forest, support vector machines, or neural networks. The goal of the model is to predict the immune response of an individual based on their gene expression profile. To build the model, you will need to first split your data into training and testing sets. The training set will be used to train the model, while the testing set will be used to evaluate its performance. You can then use the DEGs as input features and the immune response (e.g., cytokine levels) as the output variable. Once the model is trained, you can use it to predict the immune response of new individuals based on their gene expression profile. This can be useful for identifying individuals who may be at higher risk of developing severe COVID-19 or for developing personalized treatment plans.
问：What is the most import genes and useful informtion in this artical
答：This article discusses the differential expression of immune-related and antiviral genes in upper airway samples from individuals with asymptomatic or mild COVID-19 compared to healthy controls. The study found that all the studied genes were differently expressed between SARS-CoV-2 individuals and the healthy control group, except for IL1B. The first component grouped genes that were overexpressed in COVID-19 cases when compared to healthy controls, including IFI6, IRF9, and OAS1, which are interferon-stimulated genes (ISG) that play an important role in pathogen control. The study also found that individuals with high viral loads had increased expression levels of OAS1 and IFI6 genes. The results suggest a protective role of IFI6, OAS1, and IRF9 in asymptomatic-mild cases of SARS-CoV-2 infection. Therefore, the most important genes and useful information in this article are the differential expression of IFI6, IRF9, and OAS1 genes in COVID-19 cases and their potential protective role in the immune response against SARS-CoV-2 infection.
然后我想让它帮我做做PPT
问：If I need to share this literature in an academic discussion, I need to consider the content of the literature, the direction of reproduction, the explanation of professional knowledge points, and understand the logic of the article, please help me design a plan and adopt PPT format
答：Sure, here's a plan for presenting this literature in an academic discussion using a PowerPoint (PPT) format: Slide 1: Title slide - Title of the presentation - Presenter's name and affiliation
Slide 2: Introduction - Briefly introduce the topic of the presentation - Provide background information on SARS-CoV-2 and its impact on human health - Mention the purpose of the study and its significance
Slide 3: Study design - Describe the study design and methods used in the research - Mention the sample size and characteristics of the study population - Explain the statistical analysis used to identify differentially expressed genes
Slide 4: Results - Present the main findings of the study - Highlight the differentially expressed genes identified in the study, including IFI6, IRF9, and OAS1 - Explain the potential protective role of these genes in the immune response against SARS-CoV-2 infection
Slide 5: Discussion - Discuss the implications of the study's findings - Mention the limitations of the study and areas for future research - Explain how the study's findings contribute to our understanding of the immune response to SARS-CoV-2 infection
Slide 6: Conclusion - Summarize the main points of the presentation - Emphasize the importance of the study's findings for understanding the immune response to SARS-CoV-2 infection
Slide 7: References - List the references cited in the presentation You can also include additional slides with images, graphs, or tables to illustrate the study's findings and make the presentation more engaging. Make sure to use clear and concise language, and avoid overwhelming the audience with too much information on each slide.
大纲出来了，还差内容，接着就可以提问
Please fill in the content for me
结果太长就不展示了，大家可以自行试试。
2. 基于ChatGPT4的文献阅读
当然上面网页可能用的是GPT3.5模型（如果是4.0也太壕了）
其实ChatGPT4也自带上传阅读大文本功能了
新的ChatGPT4自带两种新功能，一种是接入bingGPT（本文省略）
另一种就是使用plugin插件~
我这里装了几个，交互式很强，但是估计pubmed官网已经禁止GPT访问了，直接问pubmed中的文章会显示上传失败
试了几次之后感觉Link Reader更好用
回答的答案也是原文的内容，当然也可以问一些开阔性的问题，在此不再举例~
2023年注定是计算机领域划时代的一年，所有行业都将天翻地覆，当人类现存的所有智慧都能在10秒钟之内展示在我们面前时，2023年以前的学习方法都将荡然无存~至此，留给我们的问题只有一个：How to ask beautifully?